4LSW
Crystallization and Structural Analysis of 2-Hydroxyacid Dehydrogenase from Ketogulonicigenium vulgare Y25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 1W2B |
Synchrotron site | BSRF |
Beamline | 1W2B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-10-31 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 111.202, 52.333, 65.647 |
Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.640 |
R-factor | 0.2129 |
Rwork | 0.211 |
R-free | 0.25140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ba1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.055 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.700 |
High resolution limit [Å] | 1.640 | 4.150 | 1.640 |
Number of reflections | 38014 | ||
Completeness [%] | 85.0 | 99 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 23% PEG 3350, 0.2M MgCl, 0.1M pH 7.0 HEPES, VAPOR DIFFUSION, SITTING DROP, temperature 298K |