4LS9
Structure of mycobacterial nrnA homolog reveals multifunctional nuclease activities
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.870, 86.090, 66.030 |
| Unit cell angles | 90.00, 118.37, 90.00 |
Refinement procedure
| Resolution | 34.590 - 2.200 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.22510 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.100 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 33001 | |
| Completeness [%] | 99.5 | 99.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | PEG 6000, pH 7.5, VAPOR DIFFUSION, SITTING DROP |
