4LRZ
Crystal Structure of the E.coli DhaR(N)-DhaL complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 89.766, 91.500, 93.808 |
| Unit cell angles | 84.15, 72.42, 90.01 |
Refinement procedure
| Resolution | 90.910 - 2.320 |
| R-factor | 0.2021 |
| Rwork | 0.200 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.743 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 91.000 | 50.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 5.000 | 2.320 |
| Rmerge | 0.090 | 0.046 | 0.588 |
| Number of reflections | 119216 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 97.5 | 96.6 | 92.1 |
| Redundancy | 3.9 | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch, sitting drop | 6.5 | 295 | 30% (v/v) 2-ethoxyethanol, pH 6.5, microbatch, sitting drop, temperature 295K |
