4LRX
Crystal Structure of the E.coli DhaR(N)-DhaK complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-15 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 |
Unit cell lengths | 232.129, 232.129, 79.891 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.250 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.22760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.631 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.370 |
High resolution limit [Å] | 3.250 | 7.000 | 3.250 |
Rmerge | 0.134 | 0.043 | 0.525 |
Number of reflections | 39071 | ||
<I/σ(I)> | 7.5 | ||
Completeness [%] | 99.8 | 99.8 | 99.1 |
Redundancy | 7.7 | 8.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 40% PEG 200, pH 6.5, vapor diffusion, sitting drop, temperature 295K |