4LRK
Bacterial Effector NleH2 Kinase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 77.2 |
| Detector technology | CCD |
| Collection date | 2012-05-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 145.288, 145.288, 83.456 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.944 - 2.274 |
| R-factor | 0.1758 |
| Rwork | 0.174 |
| R-free | 0.21960 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.270 |
| Number of reflections | 41406 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES 6.5, 30% PEG 5000MME, 200 mM (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES 6.5, 30% PEG 5000MME, 200 mM (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
