4LQW
Crystal structure of HIV-1 capsid N-terminal domain in complex with NUP358 cyclophilin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.180, 38.260, 123.810 |
| Unit cell angles | 90.00, 100.64, 90.00 |
Refinement procedure
| Resolution | 33.040 - 1.950 |
| R-factor | 0.20705 |
| Rwork | 0.205 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ak4 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.823 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.040 | 2.004 |
| High resolution limit [Å] | 1.953 | 1.953 |
| Number of reflections | 48326 | |
| Completeness [%] | 94.5 | 88.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 23 % v/v PEG 4000, 23 % glycerol, 8.5 % isopropanol, 85 mM HEPES pH 7.5, 20 mM spermine tetrahydrochloride, 100 mM glycine, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
