4LQS
Crystal structure of the Cbk1-Mob2 kinase-coactivator complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-30 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.430, 79.980, 117.580 |
| Unit cell angles | 90.00, 117.60, 90.00 |
Refinement procedure
| Resolution | 47.340 - 3.300 |
| R-factor | 0.272 |
| Rwork | 0.270 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.803 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 17276 | |
| Completeness [%] | 99.4 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 296 | 25% PEG 20,000 buffered with 0.1M Na-citrate, pH 5.5, Microbatch under oil, temperature 296K |
