4LQM
EGFR L858R in complex with PD168393
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 146.173, 146.173, 146.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.18672 |
| Rwork | 0.184 |
| R-free | 0.22797 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2itv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.376 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.053 | 0.477 |
| Number of reflections | 17948 | |
| <I/σ(I)> | 26.8 | 2.4 |
| Completeness [%] | 97.2 | 87.6 |
| Redundancy | 5.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 40% PEG400, 0.15M NACL, 0.1M HEPES 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
