4LQJ
Two minutes iron loaded frog M ferritin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.99999, 1.72202, 1.75866 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.548, 184.548, 184.548 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.140 - 1.200 |
| R-factor | 0.13614 |
| Rwork | 0.136 |
| R-free | 0.14494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ka3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.998 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.140 | 46.140 | 1.260 |
| High resolution limit [Å] | 1.200 | 3.790 | 1.200 |
| Rmerge | 0.064 | 0.034 | 0.409 |
| Number of reflections | 83387 | ||
| <I/σ(I)> | 16.3 | 44 | 4.7 |
| Completeness [%] | 99.6 | 99.1 | 99 |
| Redundancy | 9.2 | 9.3 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 1.6-2M magnesium chloride, 0.1M bicine , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
