4LQF
Structure of murine IgG2b A2C7-Fab in complex with vaccinia antigen A33R at the resolution of 2.3 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.280, 73.180, 221.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.760 - 2.300 |
| R-factor | 0.19897 |
| Rwork | 0.197 |
| R-free | 0.23046 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A20G2-Fv domain (reported in same publication) conserved domain of 3E5 IgG3 Fab (extracted from pdb entry 4HDI) one monomer of A33 (extracted from pdb entry 3K7B) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.006 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.760 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 | 0.644 |
| Number of reflections | 25343 | |
| <I/σ(I)> | 11.3 | 2 |
| Completeness [%] | 96.9 | 90.9 |
| Redundancy | 4.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG3000, 0.1M imidazole, 0.2M zinc acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
