4LQ8
Rickettsia rickettsii cell surface antigen 4 (sca4) head domain (residues 21-360)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 98.152, 98.152, 155.526 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.080 - 2.200 |
| R-factor | 0.1799 |
| Rwork | 0.178 |
| R-free | 0.21390 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Refinement software | BUSTER (2.13.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.075 |
| Number of reflections | 40961 |
| <I/σ(I)> | 16.2 |
| Completeness [%] | 95.4 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.8M sodium malonate (pH 6), 5% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
