4LQ6
Crystal structure of Rv3717 reveals a novel amidase from M. tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0714 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 37.350, 103.780, 129.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.090 - 1.680 |
| R-factor | 0.1617 |
| Rwork | 0.161 |
| R-free | 0.18420 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.520 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Number of reflections | 29381 | |
| Completeness [%] | 100.0 | 3.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
