4LPZ
ARNT transcription factor/coactivator complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.846, 59.816, 73.510 |
| Unit cell angles | 90.00, 97.85, 90.00 |
Refinement procedure
| Resolution | 44.268 - 3.150 |
| R-factor | 0.2472 |
| Rwork | 0.244 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eq1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.759 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.105 | |
| Number of reflections | 8354 | |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 94.9 | 65.1 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1M Succinic acid, 30% w/v D-Sorbitol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
