4LPU
Crystal structure of TENCON variant P40AR2-32R2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 84.730, 84.730, 127.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 3.400 |
| R-factor | 0.27997 |
| Rwork | 0.277 |
| R-free | 0.33517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tes |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.266 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.101 | 0.475 |
| Number of reflections | 3822 | |
| Completeness [%] | 93.8 | 65.3 |
| Redundancy | 16.4 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES, pH 6.5, 25% PEG3000, 0.2 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
