4LPT
Crystal structure of monomeric TENCON variant P54CR4-31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-06-03 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.520, 64.130, 194.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.544 |
| R-factor | 0.20112 |
| Rwork | 0.197 |
| R-free | 0.27517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBE ENTRY 3TES |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.231 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.610 |
| High resolution limit [Å] | 2.544 | 2.544 |
| Rmerge | 0.056 | 0.321 |
| Number of reflections | 21214 | |
| Completeness [%] | 98.4 | 83.9 |
| Redundancy | 13.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M acetate, pH 4.5, 19% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
