4LPN
Frog M-ferritin with cobalt, D127E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.003, 184.003, 184.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.001 - 1.660 |
| R-factor | 0.1597 |
| Rwork | 0.159 |
| R-free | 0.17680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ka4 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.169 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.079 | 0.047 | 0.486 |
| Number of reflections | 32085 | ||
| <I/σ(I)> | 16.8 | ||
| Completeness [%] | 98.3 | 99.9 | 83 |
| Redundancy | 18.6 | 19.3 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 2M MgCl2, 0.1M Bicine, 0.1M CoCl2, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
