4LPJ

One minute iron loaded frog M ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.2399
Spacegroup name	F 4 3 2
Unit cell lengths	184.300, 184.300, 184.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.280 - 1.270
R-factor	0.15159
Rwork	0.150
R-free	0.17501
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ka3
RMSD bond length	0.006
RMSD bond angle	1.165
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.630	24.630	1.350
High resolution limit [Å]	1.270	4.050	1.280
Rmerge	0.052	0.029	0.403
Number of reflections	67104
<I/σ(I)>	14.1	39.5	2
Completeness [%]	97.2	99.5	82.9
Redundancy	6.2	7.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	1.6-2M magnesium chloride, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K