4LPJ
One minute iron loaded frog M ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.2399 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.300, 184.300, 184.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.280 - 1.270 |
| R-factor | 0.15159 |
| Rwork | 0.150 |
| R-free | 0.17501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ka3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.165 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 24.630 | 24.630 | 1.350 |
| High resolution limit [Å] | 1.270 | 4.050 | 1.280 |
| Rmerge | 0.052 | 0.029 | 0.403 |
| Number of reflections | 67104 | ||
| <I/σ(I)> | 14.1 | 39.5 | 2 |
| Completeness [%] | 97.2 | 99.5 | 82.9 |
| Redundancy | 6.2 | 7.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 1.6-2M magnesium chloride, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
