4LP7
Crystal structure of the human metapneumovirus matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 31 |
| Unit cell lengths | 62.009, 62.009, 275.385 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.700 - 2.830 |
| R-factor | 0.1873 |
| Rwork | 0.185 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vqp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.530 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.5.2) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.701 | 2.990 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.194 | 1.820 |
| Number of reflections | 27947 | |
| <I/σ(I)> | 9.5 | 1.7 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 9.6 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG6000, 100 mM Tris, pH 8.0, 10 mM zinc chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
