4LP5
Crystal structure of the full-length human RAGE extracellular domain (VC1C2 fragment)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 |
| Unit cell lengths | 180.010, 180.010, 48.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.179 - 3.800 |
| R-factor | 0.2467 |
| Rwork | 0.242 |
| R-free | 0.28590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lp4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.000 | 3.900 | |
| High resolution limit [Å] | 3.800 | 20.000 | 3.800 |
| Rmerge | 0.068 | 0.034 | 0.843 |
| Number of reflections | 8902 | ||
| <I/σ(I)> | 23.23 | 12.16 | 2.94 |
| Completeness [%] | 96.4 | 96.2 | 72.3 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M Tris, 10% Ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
