4LP5

Crystal structure of the full-length human RAGE extracellular domain (VC1C2 fragment)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-23
Detector	PSI PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 65
Unit cell lengths	180.010, 180.010, 48.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.179 - 3.800
R-factor	0.2467
Rwork	0.242
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lp4
RMSD bond length	0.005
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000		3.900
High resolution limit [Å]	3.800	20.000	3.800
Rmerge	0.068	0.034	0.843
Number of reflections	8902
<I/σ(I)>	23.23	12.16	2.94
Completeness [%]	96.4	96.2	72.3
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.1 M Tris, 10% Ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K