4LOX
Crystal structure of the I-SmaMI LAGLIDADG homing endonuclease bound to cleaved DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.284, 67.429, 98.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.560 - 1.980 |
| R-factor | 0.19059 |
| Rwork | 0.186 |
| R-free | 0.27697 |
| Structure solution method | MIR |
| Starting model (for MR) | 3qqy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.691 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (MR) |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 | 2.060 |
| High resolution limit [Å] | 1.980 | 2.510 | 1.980 |
| Number of reflections | 29068 | ||
| <I/σ(I)> | 13.52 | ||
| Completeness [%] | 91.5 | 100 | 55.3 |
| Redundancy | 2.9 | 3.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG550 MME, 50mM magnesium chloride, 0.1M TrisHCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
