4LOB
Crystal structure of polyprenyl diphosphate synthase A1S_2732 (Target EFI-509223) from Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-06 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.245, 46.008, 49.286 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.500 - 2.000 |
| R-factor | 0.20231 |
| Rwork | 0.200 |
| R-free | 0.26609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jyx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.958 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 23096 | |
| <I/σ(I)> | 6.1 | 1.7 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 7.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | 0.5 M potassium thiocyanate, pH 4.5, 0.1 M sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
