4LO8
HA70(D3)-HA17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.810, 228.859, 78.718 |
| Unit cell angles | 90.00, 92.05, 90.00 |
Refinement procedure
| Resolution | 45.781 - 2.400 |
| R-factor | 0.1995 |
| Rwork | 0.197 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.760 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.125 | 0.689 |
| Number of reflections | 62548 | |
| <I/σ(I)> | 8.4 | 1.7 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.8 | 292 | 0.1 M sodium acetate, 12% PEG2000 MME, 0.1 M cesium chloride, pH 4.8, EVAPORATION, temperature 292.0K |
