4LNQ

Crystal structure of Ifi202 HINa domain in complex with 20bp dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 2
Unit cell lengths	95.353, 105.566, 65.143
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.148 - 2.000
R-factor	0.2016
Rwork	0.200
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b6y
RMSD bond length	0.008
RMSD bond angle	1.116
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.096	0.634
Number of reflections	44832
<I/σ(I)>	27.4	4.4
Completeness [%]	99.7	99.7
Redundancy	7.8	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	294	0.1 M Bis-Tris, pH 5.5, 0.2M ammonium acetate, 10mM strontium chloride and 17% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K