4LMX

Light harvesting complex PE555 from the cryptophyte Hemiselmis andersenii CCMP644

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	66.073, 76.741, 142.593
Unit cell angles	90.00, 92.86, 90.00

Refinement procedure

Resolution	18.636 - 1.800
R-factor	0.1609
Rwork	0.158
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qgw
RMSD bond length	0.006
RMSD bond angle	1.076
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_867))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	142.416	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.079	0.488
Number of reflections	131573
<I/σ(I)>	11	2.9
Completeness [%]	99.6	97.7
Redundancy	2.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	25% PEG10000, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K