4LMV
Crystal structure of glutathione transferase GSTFuA2 from Phanerochaete chrysosporium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.412, 202.466, 192.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.957 - 3.204 |
| R-factor | 0.1924 |
| Rwork | 0.189 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g19 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.536 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.957 | 3.370 |
| High resolution limit [Å] | 3.204 | 3.200 |
| Rmerge | 0.120 | 0.581 |
| Number of reflections | 32711 | |
| <I/σ(I)> | 20.7 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.2 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 5.6 | 277 | 0.1 sodium citrate, 30% w/v PEG 4000, pH 5.6, microbatch under oil, temperature 277K |
