4LMO

Structure of a vertebrate RNA binding domain of telomerase (TRBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1, 1.008
Spacegroup name	P 1 21 1
Unit cell lengths	48.629, 84.508, 137.174
Unit cell angles	90.00, 94.31, 90.00

Refinement procedure

Resolution	20.000 - 2.370
R-factor	0.22191
Rwork	0.220
R-free	0.25901
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.943
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.430
High resolution limit [Å]	2.370	2.370
Number of reflections	42409
Completeness [%]	98.6	87.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	50 mM Hepes 7.0, 40% v/v Tacsimate, pH 7.0, 0.002 M Spermine, 0.002 M Hexammine cobalt(III) chloride, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	50 mM Hepes 7.0, 40% v/v Tacsimate, pH 7.0, 0.002 M Spermine, 0.002 M Hexammine cobalt(III) chloride, VAPOR DIFFUSION, SITTING DROP, temperature 291K