4LLW
Crystal structure of Pertuzumab Clambda Fab with variable domain redesign (VRD2) at 1.95A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-02-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | .97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.596, 71.179, 90.924 |
| Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
| Resolution | 35.320 - 1.950 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.510 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.320 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.113 | 0.719 |
| Number of reflections | 76929 | |
| Completeness [%] | 97.9 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 294 | 15% PEG 8K + 200mM Ammonium Sulfate , pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
