4LLO

Structure of the eag domain-CNBHD complex of the mouse EAG1 channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 65
Unit cell lengths	162.380, 162.380, 100.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.710 - 1.995
R-factor	0.1684
Rwork	0.167
R-free	0.19740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f8a
RMSD bond length	0.007
RMSD bond angle	1.043
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1269))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.710	2.100
High resolution limit [Å]	1.995	2.000
Rmerge	0.160	0.729
Number of reflections	102374
<I/σ(I)>	9.5	2.7
Completeness [%]	100.0	100
Redundancy	11.8	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	18% (w/v) PEG 3350, 1.8% (v/v) Tacsimate, 0.01M MnCl2 and 0.09M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K