4LLI

Crystal Structure of human Myosin 5a globular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-27
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9334
Spacegroup name	P 21 21 21
Unit cell lengths	74.057, 87.105, 130.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.500 - 2.200
R-factor	0.201
Rwork	0.198
R-free	0.24600
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.023
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.500	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	43515
<I/σ(I)>		6.3
Completeness [%]	99.5	99.5
Redundancy		7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	0.112-0.125 M succinic acid pH 7.0. 16-18% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K