4LLE
The crystal structure of R60L mutant of the histidine kinase (KinB) sensor domain from Pseudomonas aeruginosa PA01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.235, 47.804, 49.422 |
| Unit cell angles | 75.98, 80.19, 74.54 |
Refinement procedure
| Resolution | 34.026 - 1.680 |
| R-factor | 0.1983 |
| Rwork | 0.196 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kkb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.924 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.065 | 0.518 |
| Number of reflections | 42068 | |
| <I/σ(I)> | 41 | 4 |
| Completeness [%] | 95.6 | 95.9 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 0.17M Ammonium acetate, 0.085M Sodium Acetate-HCl, 25.5% (w/v) PEG4000, 15% (v/v) Glycerol, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
