4LL8
Complex of carboxy terminal domain of Myo4p and She3p middle fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 272.013, 38.116, 183.991 |
| Unit cell angles | 90.00, 125.24, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.578 |
| Rwork | 0.224 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.574 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.578 |
| Number of reflections | 18820 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M HEPES, pH 7.0, 8% Tacsimate, 12% (w/v) PEG 3350, 0.01M DTT, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
