4LL6
Structure of Myo4p globular tail domain.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9798, 0.9801, 0.9574 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.881, 76.363, 77.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.300 |
| Rwork | 0.225 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 17120 |
| Completeness [%] | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% Tacsimate, 0.1M MES, pH 7.0, 25% (w/v) PEG 1K, 10mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
