4LKI

The structure of hemagglutinin L226Q mutant from a avian-origin H7N9 influenza virus (A/Anhui/1/2013)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	H 3 2
Unit cell lengths	116.095, 116.095, 295.535
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.592 - 2.700
R-factor	0.2404
Rwork	0.238
R-free	0.27650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dj6
RMSD bond length	0.006
RMSD bond angle	0.974
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	21325
Completeness [%]	99.3	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	16%(w/v) Polyethylene glycol 3350, 0.2M lithium sulfate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K