4LKG

The structure of hemagglutinin from a avian-origin H7N9 influenza virus (A/Shanghai/1/2013) in complex with avian receptor analog 3'SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 3
Unit cell lengths	110.764, 110.764, 133.227
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.586 - 2.994
R-factor	0.2472
Rwork	0.246
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dj6
RMSD bond length	0.005
RMSD bond angle	0.895
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	2.994	3.000
Number of reflections	36748
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1M sodium citrate tribasic dehydrate, 18%(w/v) Polyethylene glycol 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K