4LKD
Crystal Structure of Pseudomonas aeruginosa Lectin LecA Complexed with GalA-QRS at 2.31 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.216, 149.239, 87.018 |
| Unit cell angles | 90.00, 110.96, 90.00 |
Refinement procedure
| Resolution | 48.460 - 2.307 |
| R-factor | 0.2162 |
| Rwork | 0.215 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.672 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.461 | 2.450 |
| High resolution limit [Å] | 2.307 | 2.310 |
| Rmerge | 0.789 | |
| Number of reflections | 81750 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 98.0 | 92.9 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.5M Lithium sulfate monohydrate, 0.1M Tris pH 8.5 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
