4LK2

Crystal structure of RNA splicing effector Prp5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	87.159, 86.081, 127.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.881 - 2.120
R-factor	0.2131
Rwork	0.212
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.086
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.050
Number of reflections	59417

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M Bis-Tris propane, 20% PEG 3350, 0.2M NaF , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K