4LJY

Crystal structure of RNA splicing effector Prp5 in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	86.598, 86.598, 125.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.299 - 1.950
R-factor	0.2019
Rwork	0.201
R-free	0.22630
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.139
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.910
Number of reflections	37859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M MES, 22.5% PEG 4000, 0.6M NaCl , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K