4LJX

Crystal structure of an AT-rich interactive domain-containing protein 3A (ARID3A) from Homo sapiens at 2.21 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-22
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1
Unit cell lengths	34.924, 43.092, 44.894
Unit cell angles	108.12, 82.22, 98.17

Refinement procedure

Resolution	42.467 - 2.210
R-factor	0.1832
Rwork	0.182
R-free	0.20170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kk0
RMSD bond length	0.010
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	PHASER (2.3.0)
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.467	42.467	2.290
High resolution limit [Å]	2.210	4.740	2.210
Rmerge	0.128	0.066	0.568
Number of reflections	11361	1153	1157
<I/σ(I)>	7.31	14.7	2.5
Completeness [%]	92.0	92.6	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	20.00% PEG-6000, 0.1M Citrate pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K