4LJR
Structural insights into the unique single-stranded DNA binding mode of DNA processing protein A from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.400, 40.510, 154.680 |
| Unit cell angles | 90.00, 92.18, 90.00 |
Refinement procedure
| Resolution | 33.155 - 1.800 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ljl |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.870 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.510 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 36291 |
| Completeness [%] | 83.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 15% PEG3350, 100 mM NaAc, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
