4LJM
Crystal structure of C-terminal RNA recognition motif of human ETR3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 118.533, 118.533, 118.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.440 - 3.000 |
| R-factor | 0.18434 |
| Rwork | 0.183 |
| R-free | 0.20936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b7f |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.495 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.440 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.147 | 0.621 |
| Number of reflections | 10792 | |
| <I/σ(I)> | 10.7 | 2.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1.8M Ammonium citrate dibasic, 0.1M Sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
