4LJM

Crystal structure of C-terminal RNA recognition motif of human ETR3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-02-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 3
Unit cell lengths	118.533, 118.533, 118.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.440 - 3.000
R-factor	0.18434
Rwork	0.183
R-free	0.20936
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b7f
RMSD bond length	0.023
RMSD bond angle	2.495
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.440	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.147	0.621
Number of reflections	10792
<I/σ(I)>	10.7	2.2
Completeness [%]	99.7	100
Redundancy	4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	1.8M Ammonium citrate dibasic, 0.1M Sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K