4LJB

Structure of a photobleached state of IrisFP under high intensity laser-light

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	86.099, 96.416, 140.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.013 - 1.902
R-factor	0.3416
Rwork	0.339
R-free	0.38390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2VVH - IRISFP FLUORESCENT PROTEIN IN ITS GREEN FORM CIS CONFORMATION
RMSD bond length	0.009
RMSD bond angle	1.523
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.320	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	91193
Completeness [%]	99.2	99.2
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	2.1M ammonium sulfate, 0.1M bicine, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K