4LIW

CcmK1 Carboxysome Shell Protein from Synechocystis PCC6803, L11K Point Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-11-22
Detector	RIGAKU RAXIS HTC
Spacegroup name	P 63
Unit cell lengths	70.020, 70.020, 56.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.240 - 1.600
R-factor	0.192
Rwork	0.180
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dnc
RMSD bond length	0.012
RMSD bond angle	1.315
Data scaling software	XSCALE
Phasing software	PHASER (2.5.4)
Refinement software	PHENIX (dev_1334)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.240	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.055	0.244
Number of reflections	20387
<I/σ(I)>	34.07	5.55
Completeness [%]	98.0	84.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.5	298	0.1M N-cyclohexyl-2-aminoethanesulfonic acid, 1.26M ammonium sulfate, 0.15M sodium chloride, 0.01M guanidinium chloride, pH 9.5, vapor diffusion, hanging drop, temperature 298K