4LIN

Exploring the atomic structure and conformational flexibility of a 320 angstrom long engineered viral fiber using X-ray crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-03
Detector	ADSC QUANTUM 270
Wavelength(s)	0.92
Spacegroup name	P 1
Unit cell lengths	38.689, 147.929, 151.011
Unit cell angles	87.94, 90.05, 89.95

Refinement procedure

Resolution	14.990 - 2.700
R-factor	0.2318
Rwork	0.229
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.991
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Number of reflections	84119
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	290	20% (w/v) Polyethylene glycol 8000, 0.1M dibasic potassium phosphate, 0.1M sodium citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	4.5	290	20% (w/v) Polyethylene glycol 8000, 0.1M dibasic potassium phosphate, 0.1M sodium citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K