4LID
A100, A DNA binding scaffold from Sulfolobus spindle-shape virus 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 102.5 |
| Detector technology | PIXEL |
| Collection date | 2013-04-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 152.999, 152.999, 61.727 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.000 - 3.000 |
| R-factor | 0.23767 |
| Rwork | 0.237 |
| R-free | 0.25062 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.743 |
| Data reduction software | autoXDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.250 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.014 | 0.688 |
| Number of reflections | 8953 | |
| <I/σ(I)> | 7.93 | 5.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.5 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 296 | 10mg/ml A100, 0.6-0.9M Ammonium dihydrogen phosphate, 20-30% Glycerol, 0.7M MES, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
