4LHQ
Ricin A chain bound to camelid nanobody (VHH8)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.08 |
Spacegroup name | P 41 |
Unit cell lengths | 49.048, 49.048, 339.595 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.544 - 2.300 |
R-factor | 0.1929 |
Rwork | 0.192 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.879 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.076 | 0.049 | 0.380 |
Number of reflections | 34433 | ||
<I/σ(I)> | 9.6 | ||
Completeness [%] | 97.0 | 94.9 | 78.9 |
Redundancy | 4.9 | 6.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 293 | 100 mM NaAcetate, 2 M NaFormate, pH 4.6, VAPOR DIFFUSION, temperature 293K |