4LHP

Crystal Structure of Native FG41Malonate Semialdehyde Decarboxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-06-22
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	88.955, 94.692, 190.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.826 - 2.020
R-factor	0.196
Rwork	0.194
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aal
RMSD bond length	0.004
RMSD bond angle	0.764
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CaspR
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	91098
Completeness [%]	85.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Sitting drop	7.5	277	3 micro liter of protein solution (20.5 mg/mL in 10 mM sodium phosphate buffer, pH 8) mixed with 3 micro liter crystallization solution (0.1 M HEPES-Na buffer, pH 7.5, 2% v/v polyethylene glycol 400, 2.0 M ammonium sulfate), Sitting drop, temperature 277K