4LHN

Structure of the N-terminal domain of the Flo1 adhesin (N-Flo1p) from the yeast Saccharomyces cerevisiae, in complex with calcium and mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	298
Detector technology	CCD
Collection date	2013-05-16
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	46.700, 63.590, 106.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.030 - 2.120
R-factor	0.14838
Rwork	0.146
R-free	0.19405
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xjq
RMSD bond length	0.020
RMSD bond angle	2.086
Data reduction software	XDS
Data scaling software	Aimless ((CCP4))
Phasing software	BALBES (online server)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.370	2.230
High resolution limit [Å]	2.120	2.120
Rmerge	0.100	0.433
Number of reflections	16718
<I/σ(I)>	12.3	3.7
Completeness [%]	90.1	80.3
Redundancy	5.7	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	Protein solution: 50 mM sodium acetate pH 5, 150 mM NaCl, 10 mM CaCl2 Crystallization condition: 1 M NaCl, 15% v/v EtOH, VAPOR DIFFUSION, HANGING DROP, temperature 293K