4LHK
Structure of the N-terminal domain of the Lg-Flo1 adhesin (N-Lg-Flo1p) from the yeast Saccharomyces pastorianus, in complex with calcium and alpha-1,2-mannobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.618, 85.476, 68.240 |
| Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
| Resolution | 35.130 - 1.730 |
| R-factor | 0.14023 |
| Rwork | 0.137 |
| R-free | 0.19329 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xjq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.829 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES (online server) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.540 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.092 | 0.704 |
| Number of reflections | 44852 | |
| <I/σ(I)> | 9.9 | 2.1 |
| Completeness [%] | 97.3 | 96 |
| Redundancy | 4.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Protein solution: 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 10 mM CaCl2 Crystallization condition: 0.1 M amino acids (L-Na-glutamate, DL-alanine, glycine, DL-lysine-HCl, DL-serine), 0.1 M imidazole-MES pH 6.5, 30% w/v PEG 550 MME - PEG 20000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
