4LHF

Crystal structure of a DNA binding protein from phage P2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 65
Unit cell lengths	66.254, 66.254, 33.802
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.127 - 2.401
R-factor	0.211
Rwork	0.209
R-free	0.25280
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.733
Data scaling software	SCALA (3.2.25)
Phasing software	SOLVE
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.378	33.847	2.530
High resolution limit [Å]	2.401	7.590	2.400
Rmerge	0.077	0.042	0.479
Total number of observations		827	1447
Number of reflections	3108
<I/σ(I)>	17.3	12.7	1.6
Completeness [%]	92.9	92.7	86.1
Redundancy	6	7.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.2 M Na-citrate pH 5.0, 24% PEG 4000, 20% isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.2 M Na-citrate pH 5.0, 24% PEG 4000, 20% isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K