4LGX
Structure of Chitinase D from Serratia proteamaculans revealed an unusually constrained substrate binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-04-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.330, 87.156, 59.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.190 - 1.490 |
| R-factor | 0.1631 |
| Rwork | 0.162 |
| R-free | 0.17649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qok |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.627 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.190 | 1.520 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Number of reflections | 64748 | |
| <I/σ(I)> | 44.5 | 6.1 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2M SODIUM FORMATE, 0.1M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
